Figure 1
From: A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors
Construction and characterization of the CD19-IgG 1 Fc fusion proteins. A. Diagrams of the lentiviral vector provirus constructs used to transduce the CD19sIg fusion genes. B. Reduced protein electrophoresis by SDS PAGE of fusion protein products after concentration and purification using Protein A Dynabeads. Centricon concentrated supernatant samples from fresh Pro293a™-CDM (A), 293 T cells (B), 293 T cells expressing CD19sIg1-3 (C), and 293 T cells expressing CD19sIg1-4 (D). Lanes E and F are the DYNAL purified extracts for CD19sIg1-3 and CD19sIg1-4 (respectively). Arrows indicate the predicted size of the monomer, 47 kDa for CD19sIg1-3 and 57 kDa for CD19sIg1-4. All samples were reduced prior to loading. C. Native protein electrophoresis of CD19sIg1-4 after concentration and purification using Protein A Dynabeads; arrow points to 171 kDa band (expected size for trimers of the fusion protein). D. Results of a comparative ELISA using FMC63 monoclonal capture antibody, of purified fusion proteins, in native (N) or denatured (D) forms. E. Results of ELISA using antibodies targeting human CD19 molecule (FMC63, HIB19, F-3 and 2E2B6B10), human CD20 (B9E9) and PSMA (YPSMA-1).