The levels of phosphorylated-ERK1/2 (pERK1/2), AT1 receptor, Ang II and Ang-(1–7) in mice. (A−B), The phosphorylated level of extracellular regulated kinase1/2 (ERK1/2; A) and protein level of AT1 receptor (B) were measured by Western blotting analysis in the Ace2+/y control mice and Ace2-/y mice treated with irbesartan (Irb) or saline (control). n = 5 for each group. (C−E), Plasma levels of Ang II (C) and Ang-(1−7) (D) and the ratio of Ang-(1−7)/Ang II (E) in mice. n = 6 for the Ace2+/y control group and Ace2-/y irbesartan group; n = 8 for the Ace2-/y control group. **P < 0.01 compared with Ace2+/y control group; #, P < 0.05, ##, P < 0.01 compared with Ace2-/y control group.