Cardiac protein levels of CTGF and collagen in mice. Representative Western blot (A) exhibited cardiac protein levels of CTGF (B), collagen I (C) and collagen III (D) in mice. α-tubulin was used as endogenous control. R.E. = relative expression; CTGF, connective tissue growth factor; Irb, irbesartan. n = 5 for each group. **P < 0.01 compared with Ace2+/y control group; #, P < 0.05 compared with Ace2-/y control group.