Cardiac mRNA levels of hypertrophy and fibrosis related genes in mice. TaqMan real-time PCR analysis revealed mRNA expression of hypertrophy and fibrosis related genes including ANF (A), β-MHC (B), TGFβ1 (C), and fibronectin (D) in the hearts of mice. 18S rRNA was used as an endogenous control. R.E. = relative expression; ANF, atrial natriuretic factor; β-MHC, β-myosin heavy chain; TGFβ1, transforming growth factor-β1. n = 6 for the Ace2+/y control group and Ace2-/y irbesartan group; n = 8 for the Ace2-/y control group. **P < 0.01 compared with Ace2+/y control group; ##, P < 0.01 compared with Ace2-/y control group.