H19 was up-regulated in gastric cancer tissues, gastric cancer cell lines and other common cancer cell lines. Real-time qRT-PCR was used to determine the expression level of H19. The ∆Ct value was determined by subtracting the β-actin Ct value from the target lncRNA Ct value. Smaller ∆Ct value indicates higher expression. Level of H19 in gastric cancer biopsy tissues (n = 15, P = 0.014; A) and gastric cancer tissues (n = 77, P = 0.029; B). Sequencing result of qRT-PCR product of H19 (C). The level of H19 in gastric cancer cell lines (AGS, MGC-803 and SGC-7901) and liver cancer cell lines (SMMC-7721 and HepG2) was higher than that in human gastric epithelial cell line GES-1 and human liver normal cell line HL-7702, respectively; however, its level in lung cancer cell line A549 and prostate cancer cell lines (Du-145 and PC-3) was lower than that in human fetal lung fibroblast cell line HELF cells and human prostate epithelial cell line RWPE-1 (D). All results were expressed as the Means ± SD of three independent experiments. #P < 0.001.