Representative fluorescence photographs of SATB1 and PCNA protein expression in various NPC cell lines and NP-69 cells. Cells were grown on glass coverslips for 48 hours and incubated with primary rabbit-anti human SATB1 and mouse-anti human PCNA antibodies overnight. The antigenic sites of SATB1 and PCNA were detected using FITC-, TRITC-conjugated goat anti-rabbit and anti-mouse IgG and analyzed by confocal microscopy. And cells were countstained with DAPI. Scale bars = 60 μM.