R28-GBM bearing mice treated with Ad-Delo3-RGD showed prolonged survival without any sign of toxicity. (A) Survival data of R28 CSC-derived GBM bearing mice treated as indicated are plotted using the Kaplan-Meier method. p-values were determined using the log-rank test. R28-GBM bearing mice treated with Ad-Delo3-RGD or Ad-Delo3-RGD plus TMZ survived significantly longer than mice treated with TMZ or PBS alone. (PBS vs. Ad-Delo3-RGD, p = 0.007; PBS vs. TMZ + Ad-Delo3-RGD, p = 0.013; TMZ vs. Ad-Delo3-RGD, p = 0.023; TMZ vs. TMZ + Ad-Delo3-RGD, p = 0.035. (B) Histopathological analyses of tumors (upper panel) and of non-neoplastic tumor adjacent brain tissue of Ad-Delo3-RGD-treated mice (lower panel). Upper left: PBS-treatment; upper middle: TMZ-treatment; upper right Ad-Delo3-RGD-injection. Lower panel: Ad-Delo3-RGD shows no toxicity in any non-neoplastic part of the brain including subventricula zone (left), cerebellum (middle) and cortex (right). 5 μm sections were stained with H&E.