Figure 4

Immune-related gene expression in peritoneal lavage from treated and control mice with ID8 tumor. A, Quantitative RT-PCR data demonstrating upregulated transcription of the Th1-related molecules Tbx21, Eomes, IFN-γ and Stat4 and downregulated transcription of the Th2 molecules IL-4, Stat6, and GATA3 in 2 mAb-treated mice. B, Quantitative RT-PCR data demonstrating elevation of the Th1-associated chemokine/receptor CXCL9-11/CXCR3 and decrease of the B-cell chemotactic chemokine/receptor CXCL13/CXCR5 in cells of peritoneal lavage from 2 mAb-treated mice. C, Results from quantitative RT-PCR demonstrating that mRNA for CTLA-4, PD-1, PD-L1, TIM-3, IL-10, TGF-β and FoxP3 was significantly decreased in cells of peritoneal lavage from 2 mAb-treated mice. D, IL-2 and IFN-γ production in the supernatants from pooled lavage cells harvested from treated mice which were stimulated in vitro with 50 ng/ml PMA and 1 μg/ml ionomycin for 6 hours prior to analysis. *P < 0.05, **P < 0.01, ***P < 0.001, compared with control mAb- or single mAb-treated mice.