Figure 3

Analysis of tumor-infiltrating immune cells (TIIC) in peritoneal lavage from treated mice. Mice (5/group) injected i.p. with 1 × 10⁶ ID8 cells 10 day earlier were injected thrice at 4 days interval with 250 μg of control or anti-TIM-3/CD137 mAb. Seven days later, peritoneal lavage from treated mice was analyzed by flow cytometry for the percentages and numbers of various subsets of TIIC. The percentages of CD8⁺, CD4⁺, CD4⁺FoxP3⁺ Treg and CD11b⁺GR-1⁺ MDSC in peritoneal lavage are shown in A, B, C and D respectively with each dot representing data from each mouse. The ratios of CD8⁺ and CD4⁺ T cells to Treg and MDSC in peritoneal lavage are shown in E, F, G and H respectively with each dot representing data from each mouse. The absolute numbers of CD8⁺, CD4⁺, Treg and MDSC in peritoneal lavage are shown in I, J, K and L respectively with each dot representing data from each mouse. Data are representative of 2 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.