Figure 9

Array-CGH profiles of the DNA of a papillary RCC and the respective CNHCs. The DNA of the papillary RCC of patient #37 shows gains at chromosomes 6p, 7, 16, 17q, 20 and loss of chromosome Y, changes typically found in pap. RCC (red profile). However, the array-CGH profiles of three CNHC-MF clusters (photographs A-C) are balanced (profiles A-C), whereas losses at chromosomes 3p and 4q are identified in the DNA of a CNHC-UMF cluster (photograph D and profile D, respectively) isolated from the same patient. The two clusters shown in photograph A and D are negative for CD45. All other clusters were hematoxylin stained. As a control, the DNA of an isolated pool of 10 leucocytes from blood of a healthy individual showed a balanced genome (leucocyte control). Gains and losses of the X- and Y-chromosomes (profile B and leucocyte control) do not reflect true copy number variations. They result from differences between the sex of the reference and the samples DNA (i.e. male patient DNA was hybridized against a female reference DNA thereby resulting in a loss of X and gain of Y chromosome). Bars above the x-axis are considered to be gains, below the x-axis losses of DNA.