Figure 4
Silencing effect of stathmin shRNA on gastric cancer xenograft in nude mice. MKN-45 cells were transfected with stathmin1 (STMN1) shRNA or, Non-silencing short hairpin RNA (shRNA) and nude mice were inoculated subcutaneously with 2 × 106 cells at two sites per mouse. The tumor mass (xenograft) volume was measured every week from week 3 to week 7. Data are expressed as the (Means ± S.D) and represent six independent experiments. (P < 0.05 vs Non-silencing shRNA, one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison). (A) Photograph of xenografts dissected from nude mice after 7 weeks subcutaneous inoculation showing suppression growth of cancer cells transfected with stathmin1 shRNA2 rather than non-treated control group. (B) Tumor growth curve showing a significant growth tendency in non-infected group (control) as compared to the stathmin shRNA2 transfected group (p < 0.05).