Figure 7
Morphometric analyses of mechanisms and pathways in rAAV-transduced chondrocytes in situ . Explants were transduced with rAAV-lacZ or rAAV-hTGF-β as described in Figure 1 and maintained in culture for up to 90 days. The samples were then fixed and histologically processed at the denoted time points to monitor the % of cells immunostained for MMP-13 (A), TIMP-1 (B), TIMP-3 (C), PTHrP (D), β-catenin (E), and the TGF-β receptor I (ALK1 and ALK5) (F and G, respectively). Data on the ALK1/ALK5 ratio are presented in (H).