Figure 5From: rAAV-mediated overexpression of TGF-β stably restructures human osteoarthritic articular cartilage in situEffects of rAAV-mediated TGF-β expression upon the anabolic activities of human normal and OA chondrocytes in situ . Explants were transduced with rAAV-lacZ or rAAV-hTGF-β as described in Figure 1 and maintained in culture for 90 days. The samples were then fixed and histologically processed for safranin O staining (A) and immunohistochemical detection of type-II (B) and type-X collagen (C) (all at magnification x10; view of the middle zone).Back to article page