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Table 2 Renal function, histopathology and molecular biology parameters in the kidney

From: Innate immunity activation involved in unprotected porcine auto-transplant kidneys preserved by naked caspase-3 siRNA

Detected parameters PostN PostCS PostCS+siRNA P value* PostTx PostTx+siRNA P value*
Caspase-3 mRNA Not detected 2.22 ± 0.64 0.41 ± 0.12 0.017 3.77 ± 1.08 12.16 ± 3.70 0.042
32 kD caspase-3 0.50 ± 0.08 2.33 ± 0.28 1.11 ± 0.14 0.005 0.94 ± 0.08 0.27 ± 0.04 0.002
17 kD caspase-3 0.09 ± 0.01 Non-detectable Non-detectable   0.07 ± 0.02 0.20 ± 0.06 0.026
SCr (μmol/L) 37.5 ± 8.1 76.83 ± 9.49 80.67 ± 7.10 0.753 506.4 ± 122.2 659.2 ± 49.1 0.273
BUN (mmol/L) 2.8 ± 0.4 8.05 ± 1.20 8.32 ± 0.95 0.865 35.9 ± 7.8 51.2 ± 6.5 0.164
Score of TID/200× field Not detected 9.71 ± 0.56 7.93 ± 0.84 0.093 9.94 ± 0.53 11.90 ± 0.21 0.011
Active caspase-3+ cells/400× field 0.01 ± 0.00 1.05 ± 0.15 0.32 ± 0.10 0.004 2.96 ± 0.66 6.83 ± 1.26 0.017
ISEL+ cells /400× field 0.02 ± 0.01 0.30 ± 0.06 0.36 ± 0.19 0.734 1.39 ± 0.29 6.06 ± 1.37 0.004
MPO+ cells /400× field 0.77 ± 0.34 0.06 ± 0.04 0.08 ± 0.05 0.921 15.92 ± 3.84 52.33 ± 13.31 0.028
  1. PostN, post-nephretomy; PostCS, post cold storage; PostTx, post-transplant; SCr, serum creatinine; BUN, blood urine nitrogen; TID, tubulointerstitial damage; ISEL, in situ end-labeling; MPO, myeloperoxidase. Data were presented as Mean ± SEM. *: The difference calculated between the non-preserved and siRNA-preserved kidney. The caspase-3 mRNA in the non-transplanted (PostN) control kidney was defined as baseline control using a 2−ΔΔ Ct method.