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Table 2 Renal function, histopathology and molecular biology parameters in the kidney

From: Innate immunity activation involved in unprotected porcine auto-transplant kidneys preserved by naked caspase-3 siRNA

Detected parameters

PostN

PostCS

PostCS+siRNA

P value*

PostTx

PostTx+siRNA

P value*

Caspase-3 mRNA

Not detected

2.22 ± 0.64

0.41 ± 0.12

0.017

3.77 ± 1.08

12.16 ± 3.70

0.042

32 kD caspase-3

0.50 ± 0.08

2.33 ± 0.28

1.11 ± 0.14

0.005

0.94 ± 0.08

0.27 ± 0.04

0.002

17 kD caspase-3

0.09 ± 0.01

Non-detectable

Non-detectable

 

0.07 ± 0.02

0.20 ± 0.06

0.026

SCr (μmol/L)

37.5 ± 8.1

76.83 ± 9.49

80.67 ± 7.10

0.753

506.4 ± 122.2

659.2 ± 49.1

0.273

BUN (mmol/L)

2.8 ± 0.4

8.05 ± 1.20

8.32 ± 0.95

0.865

35.9 ± 7.8

51.2 ± 6.5

0.164

Score of TID/200× field

Not detected

9.71 ± 0.56

7.93 ± 0.84

0.093

9.94 ± 0.53

11.90 ± 0.21

0.011

Active caspase-3+ cells/400× field

0.01 ± 0.00

1.05 ± 0.15

0.32 ± 0.10

0.004

2.96 ± 0.66

6.83 ± 1.26

0.017

ISEL+ cells /400× field

0.02 ± 0.01

0.30 ± 0.06

0.36 ± 0.19

0.734

1.39 ± 0.29

6.06 ± 1.37

0.004

MPO+ cells /400× field

0.77 ± 0.34

0.06 ± 0.04

0.08 ± 0.05

0.921

15.92 ± 3.84

52.33 ± 13.31

0.028

  1. PostN, post-nephretomy; PostCS, post cold storage; PostTx, post-transplant; SCr, serum creatinine; BUN, blood urine nitrogen; TID, tubulointerstitial damage; ISEL, in situ end-labeling; MPO, myeloperoxidase. Data were presented as Mean ± SEM. *: The difference calculated between the non-preserved and siRNA-preserved kidney. The caspase-3 mRNA in the non-transplanted (PostN) control kidney was defined as baseline control using a 2−ΔΔ Ct method.