The changes of AT
R, p-ERK1/2, p- p38-MAPK, and p-JNK levels in cardiac-specific M
-mAChR overexpression mice. (a) Western blot analysis of angiotensin II type 1 receptor (AT1 R) protein level in WT and TG mice treated with vehicle or Ang II (0.6 mg/kg per day) for 14 days. The increased phospho-extracellular regulated protein kinase (p-ERK) (b), phospho-p-p38 (p-p38) level (c) and phospho- c-Jun N-terminal kinases (p-JNK) level (d) induced by angiotensin II (Ang II) infusion were attenuated in TG mice. Phospho-protein levels were relative to total protein levels, while total protein levels were relative to GAPDH. GAPDH served as loading control. Values were expressed as mean ± SEM (n = 6) and normalized to the CTRL group. Values were expressed as means ± SEM. *P < 0.05 vs. WT-CTRL group; #P < 0.05 vs. WT-Ang II group. WT-CTRL, vehicle-infused wild type (WT) mice; WT-Ang II, Ang II -infused WT mice; TG-CTRL, vehicle-infused transgenic (TG) mice; TG-Ang II, Ang II-infused TG mice.