The effects of M
-mAChR overexpression on Ang II-induced cardiac hypertrophy in cardiac-specific M
-mAChR overexpression mice. (a) Representative hearts from WT and M3-mAChR overexpression transgenic (TG) mice treated with vehicle or angiotensin II (Ang II) (0.6 mg/kg per day) for 14 days. (b, c) The ratio of heart weight (HW) and left ventricle weight (LVW) to body weight (BW). (d) Hematoxylin-Eosin (HE) stained sections of representative hearts. (e, f) The mRNA expression of atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC). Values were expressed as means ± SEM (n = 6) and normalized to the WT-CTRL group, *P < 0.05 vs. WT-CTRL group; **P < 0.01 vs. WT-CTRL group; #P < 0.05 vs. WT-Ang II group. WT-CTRL, vehicle-infused wild type (WT) mice; WT-Ang II, Ang II-infused WT mice; TG-CTRL, vehicle-infused transgenic (TG) mice; TG-Ang II, Ang II-infused TG mice.