MMP-2 secretion from SMC. Equal densities of SMC were cultured in basal media (0.4% FCS) in the presence or absence of 100 nM TPA for 48 h. Conditioned media were analysed for MMP-2 secretion using gelatin zymography. (A) Mean densitometry data from SMC from freshly isolated tissue, VEH and CCE-pre-treated vessels (all n = 3, ns = non significant, *P < 0.05, **P < 0.01, **P < 0.001). (B) Representative zymogram. (C) Mean densitometry data from human SV and AAA-SMC (all n = 12, *P < 0.05, ***P < 0.001). (D) Representative zymogram.