SMC senescence. Cells were cultured in FGM for 72 h before senescent cells were detected by β-galactosidase staining at pH 6.0. (A) Representative images of PCA-SMC (blue precipitate is observed in senescent cells). Scale bar = 200 μm (n = 3). (B) Senescence score in PCA-SMC (n = 3). (C) Representative images of human SV-and AAA-SMC, scale bar = 200 μm (n = 4). (D) Senescence score in human SMC (n = 4). *P < 0.05, ***P < 0.001.