SMC apoptosis. Cells were cultured in FGM ±50 nmol/L staurosporine for 24 h. (A) Representative images of PCA-SMC (green fluorescence indicates apoptotic cells). Scale bar = 300 μm. (B) Quantification of apoptosis index in PCA-SMC (n = 3). (C) Representative images of human SV- and AAA-SMC, scale bar = 300 μm. (D) Quantification of apoptosis index in human SMC (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001, ns = non-significant.