Human SMC morphology. Cells were explanted from human aneurysmal wall tissue (AAA), or human saphenous vein (SV) and maintained in culture in full growth medium. (A) Immunocytochemical staining of α-SMA (green) and SM-MHC (red) and co-localisation in human AAA SMC. Magnification ×400, scale bar = 100 μm. (B) Representative phase contrast images of aortic SMC, (C) SV-SMC and (D) human AAA-SMC, scale bar = 100 μm. (E) The mean cell areas of 100 individual cells per patient were quantified using Image J. All n = 12, ***P < 0.001.