Figure 3

Activation of murine dendritic cells by UV-Tianjin. A to C, The expressions of CD40, CD80 and CD86 were measured by flow cytometry after 48 h of coculture with UV-Tianjin or live virus. Murine dendritic cells were dose-dependently maturated by virus particles to express CD40, CD80 and CD86, showing that UV-Tianjin retained the immunogenicity of live Tianjin strain. D, IL-6 secretion was measured in dendritic cell supernatants by ELISA at 48 h after coculture with UV-Tianjin or live virus. Amounts of IL-6 were slightly diminished after UV-Tianjin stimulation compared with corresponding dose of live virus. Columns represent mean value of triplicate samples and bars represent SD. Representative of three independent experiments.