Figure 3

Elevated levels of FOXM1 correlate with resistance to docetaxel in gastric cancer. (A) AGS, SGC-7901 and MKN-28 cells were treated with 0.02 mg/L of docetaxel for 0, 24, 48 and 72 h. MTT assay was performed to test the cell viability. The IC50 calculations in the three cell lines were 0.034 mg/L, 0.025 mg/L and 0.016 mg/L separately (D left). (B) The expression of FOXM1 in AGS cell lines with the transfection of FOXM1-siRNA, pcDNA3,1 or pcDNA3, 1-FOXM1, analyzed by western blot 48 h later. (C) Gastric cell lines AGS were treated with docetaxel at the concentration of 0.02 mg/L after FOXM1-siRNA or pcDNA3, 1-FOXM1 transfection. Cell growth curves were drawn by MTT assays. The IC50 in FOXM1 knockdown, overexpressed and control groups was 0.012 mg/L, 0.025 mg/L and 0.038 mg/L respectively (D right). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 significant.