Figure 4

Z-VAD-FMK blocks LK-A-induced apoptosis. (A) Flow cytometry analysis of CNE1 and CNE2 cells treated with LK-A (3.12 μΜ) with or without Z-VAD-FMK for 48 hrs. (B) Bar chart showing that the proportion of total apoptotic cells decreased significantly after treatment with LK-A plus Z-VAD-FMK compared to LK-A alone. Data are shown as the mean ± SD from two independent experiments (*p < 0.05). (C) Western blotting analysis of CNE1 and CNE2 cells treated with LK-A (3.12 μΜ) with or without Z-VAD-FMK (20 μΜ) for 48 hrs.