Figure 2

Centrifugation improves MV infectivity of MSC. (A) MV-GFP and hMSC were centrifuged at the speeds and times indicated prior to a 2 h incubation period. Percentage of GFP positive cells were analyzed 48 h later. (B, C) Percentage of MV-GFP infected MSC from healthy donors (440, 493B, 251) or ovarian cancer patients (FB6, FB7) at MOI of 2 (B) or 4 (C). 1000 xg centrifugation for 10 mins. Representative data from duplicate experiments.