Marker expression in TSGs derived from HRPCa-2. In control TSGs derived from HRPCa-2, we observed: (A-B) nuclear staining of AR; (C-D) cytoplasmic staining of PSA; and (E-F) cytoplasmic staining of K18 in cancer cells. Positive staining of AMACR (G-H), and negative staining of the basal epithelial cell marker p63 (G-H) and ERG (I-J) in cancer cells was also observed. ERG staining was present in endothelial cells (I-J). In CR-TSGs derived from HRPCa-2, cancer cells showed moderate nuclear staining for AR (O-P), little or no staining of PSA (Q-R), and intense cytoplasmic staining for K18 (S-T). Cancer cells were also positive for AMACR (U-V) and negative for ERG (W-X). In HRPCa-1 and TSGs derived from it, we observed strong staining for ERG (K-L) and AMACR (M-N) in cancer cells. (B), (D), (F), (H), (J), (P), (R), (T), (V), and (X) are higher magnifications of boxed areas in (A), (C), (E), (G), (I), (O), (Q), (S), (U) and (W), respectively.