Generation of TSGs from HRPCa-1 and −2. (A) Kidneys with TSGs under the capsule harvested from RAG2−/−γc−/− or NIH III mice; (B) both the graft weight and proliferation index of TSGs maintained in RAG2−/−γc−/− mice were significantly higher than those maintained in NIH III mice by Student’s t-test; (C-H) immunohistochemistry of TSGs in RAG2−/−γc−/− mice using antibodies against AMACR/p63 (C), Ki67 (D), human-specific CD31 (E and G), and human-specific Ku70 (F and H). (E-F) are control TSGs and (G-H) are TSGs one month after castration. Arrows in (D) and (E and G) point to Ki67- and CD31-positive cells, respectively. White dotted lines in (F) and (H) mark the boundary between mouse kidney and TSG. Magnification for (C) and (D) is 10X and (E-H) is 40X.