Skip to main content

Advertisement

Table 4 Immunophenotype precision

From: Validation of analytical methods in compliance with good manufacturing practice: a practical approach

BM MSCs Markers CV% Inter-experiment
CD90 FITC 6.22
CD73 PE 0.95
CD105 APC 1.86
CD45-34-14 FITC 3.13
HLADR PE 4.46
CD19 APC 3.84
iDCs Markers CV% Inter-experiment
CD83 FITC 2.2
CD40 PE 10
HLADR PERCPCY5 4.6
CD11c APC 2.7
CD80 FITC 8.4
CD86 PE 7.1
mDCs Markers CV% Inter-experiment
CD83 FITC 3.6
CD40 PE 7.9
HLADR PERCPCY5 6.3
CD11c APC 5.4
CD80 FITC 5.1
CD86 PE 3.9
CTL-LS Markers CV% Inter-experiment
CD3 FITC 9.7
CD8 PE 10
CD4 APC 8.1
CD45 PERCP 8.1
CD56 PE 7.0
CD19 APC 8.0
CTL-E Markers CV% Inter-experiment
CD45RA FITC 9.4
CD45RO PE 10
CD4 APC 3.7
CD3 PERCP 6.9
CD8 APC 6.4
  1. The immunophenotype test was performed three times under the same operating conditions by the same operator on our CTPs. The inter experiment CV% was calculated considering the mean and SD of the mean fluorescence intensity (MFI) of each cytofluorimetric marker.
  2. The acceptance criteria was an inter-experiment CV% ≤ 10%.
  3. Abbreviations: CTL LS Cytotoxic T Lymphocyte subpopulations, CTL E Cytotoxic T Lymphocyte effectors.