Overexpression of FLOT2 mRNA and protein in breast cancer tissues. (A) Representative images of Western blotting analyses of FLOT2 protein expression in four matched pairs of breast cancer (T) and adjacent noncancerous tissues (N). P84 was the loading control. (B) Average T/N ratios of FLOT2 mRNA expression in paired breast cancer (T) and adjacent noncancerous tissues (N) were quantified by qPCR and normalized against GAPDH. Error bars represent the standard deviation of the mean (SD) calculated from three parallel experiments. (C) Immunohistochemical assay of FLOT2 protein expression in four pairs of matched breast cancer tissues. *p < 0.05.