BET action on differentiating myoblasts. A. Graphical representation of myogenesis and simplified design of experimental procedures: myoblasts were differentiated in presence of 10 mM BET, differentiation progression was evaluated at early, intermediate and late phase. B. Representative bands of analyzed proteins by Western blot are shown. C-D. Myf5 and Myog immunoblots indicated that BET could regulate MRFs kinetics synthesis, accelerating differentiation progression. E-F-G. Western blot analysis showed that BET significantly enhanced MyHC, N-cad and α act protein content at 72 and 96 h. H-I-J. Western blot studies revealed that, in respect to control, BET 10 mM improved Pro IGF-1 R levels at 24- 48 h, IGF-1 R amount during all phase of differentiation and AKT levels at 72- 96 h. K. MyHC immunofluorescence analysis at 48 h indicated that BET could influence neo myotubes features, promoting the acquisition of elongated morphology. Data, obtained from three independent experiments, are expressed as fold changes (FC) mean ± SD. Significance: a = p ≤ 0.05, b = p ≤ 0.04, c = p ≤ 0.03, d = p ≤ 0.02, e = p ≤ 0.01 and f = p ≤ 0.003. Scale bar: 200 μm.