Figure 2

BET action on neo myotubes features. A. Graphical representation of myogenesis and s implified design of experimental procedures: neo myotubes were treated for 30 min, 4 h, 8 h and 24 h with different concentrations of BET (1 mM, 5 mM and 10 mM). B. Representative blot of MyHC protein expression at the indicated times. C. Western blot analysis: 10 mM BET raised MyHC protein levels, 1 mM and 5 mM BET did not change MyHC content. D. Immunofluorescence analysis of Myf6 and MyHC shows that 10 mM BET stimulated myobutes are characterized by nuclei organization to form a ring (yellow indicator). The same morphological features are evident in N-cad, α act and IGF-1 immunofluorescence studies. E. Myotubes length determination: BET myotubes are significant longer than DM myotubes. Data, obtained from three independent experiments, are expressed as fold changes (FC) mean ± SD. Significance: a = p ≤ 0.05, b = p ≤ 0.04, c = p ≤ 0.03, d = p ≤ 0.02, e = p ≤ 0.01 and f = p ≤ 0.004. Scale bar: 200 μm.