BET dose–response study. A. Graphical representation of myogenesis and simplified design of experimental procedures: neo myotubes were treated for 24 h with different concentrations of BET: 1 mM, 5 mM and 10 mM. B. Images obtained by brightfield microscopy at the end of experiment (96 h). C. Insulin-IGF-1 gene expression profiles after 24 h of BET stimuli. D. Analysis of IGF-1 R mRNA: 10 mM BET significantly increased IGF-1 R mRNA content. E. Immunoblots representative bands of Pro IGF-1 R and IGF-1 R proteins. F.-G. Western blot analysis revealed that 10 mM BET improved showing Pro IGF-1 R and IGF-1 R protein levels. We performed three independent experiments. For gene expression analysis data as presented in as fold-change (ΔΔCt) of threshold cycle (Ct) mean ± SD. For immunoblots studies, results are expressed fold changes (FC) mean ± SD. Significance: a = p ≤ 0.05, b = p ≤ 0.04, c = p ≤ 0.03, d = p ≤ 0.02, e = p ≤ 0.01 and f = p ≤ 0.004. Scale bar: 200 μm.