Figure 5

MT2A mediated IκB-α transcript up-regulation to interfere NF-κB inactivation. A, Increased IκB-α expression was induced by MT2A in BGC823 cells by immunofluorescence analysis. B, MT2A activated the promoter of IκB-α. 5.6-fold increase of IκB-α promoter luciferase activity was detected in MT2A group than that in Vector group. Data were presented relative to cells transfected with empty vector. Results represent mean ± S.D. for three independent experiments. Positive control means “PGL3-control (100 ng) + PRL-SV40 (10 ng)” plasmids are co-transfected in GC cells, and the high level of luciferase data will be detected and which evaluates the whole system of dual-luciferase activity. C, The nuclear translocation of NF-κB probe was decreased in BGC823 cells re-expressing MT2A by EMSA assay. D, The nuclear location of p65, as the main subunit of NF-κB, was reduced, and increased p65 exhibited in cytoplasm.