Figure 4

MT2A was involved in regulating IκB-α expression in gastric cells. MT2A positively regulated IκB-α expression. A, MT2A played a role in increasing IκB-α mRNA expression and reducing cyclin D1 mRNA expression in the NF-κB signaling but did not change p65 mRNA level in GC cells (“-”, parent cells). B, Western blot analysis showed that the level of MT2A expression was up-regulated and accompanied with elevation of IκB-α expression as well as decreased p-IκB-α and cyclin D1 in GC cells re-expressing MT2A. C, Silencing of MT2A in GES1 cells resulted in increased levels of cyclin D1 mRNA expression, as well as down-regulation of MT2A and IκB-α mRNA expression. D, cyclin D1 and p-IκB-α protein levels were increased by silencing of MT2A expression, accompanied with down-regulation of IκB-α expression.