Figure 1

Absent or decreased expression of MT2A is detected in cell lines and primary tumors of GC. A, MT2A mRNA level was measured by RT-PCR in GC cell lines and the normal immortal cell line-GES1. And semi-quantification analyses were performed with Imagetool 3.0 gray scale scanning software (normalized to β-actin levels). The experiment was done in triplicate. B, MT2A protein level was determined by Western blot analysis in GC cell lines and the normal immortal cell line-GES1. The MT2A protein bands were scanned (normalized to β-actin levels). C, The differential expression of MT2A mRNA transcript in 36 paired GC tissues and adjacent normal tissues. The MT2A mRNA bands were scanned (normalized to β-actin levels). D, MT2A protein levels were detected in the 36 paired GC tissues by Western blotting. The MT2A protein bands were scanned (normalized to β-actin levels). E, Decreased MT2A was observed during gastric malignant transformation. (a) intense staining of MT2A in normal tissues, (b) moderate staining of MT2A in IM tissues, (c) weak staining of MT2A in moderately differentiated primary gastric cancers and (d) negative expression of MT2A in poorly differentiated GCs (original magnification × 400). (D) Compared with the normal and precancerous lesions-IM samples, reduced MT2A was a common molecular event in gastric carcinogenesis (P < 0.001) F: Differential expression of MT2A in GC, IM and normal tissues by IHC staining. Negative: score 0; Weak: score 1-4; Strong: score 5-12. For details, see the Additional file1.