Figure 1
From: MiRNA-329 targeting E2F1 inhibits cell proliferation in glioma cells
Expression analysis of miR-329 in glioma cell lines and glioma tissues. A ) Real-time PCR analysis of miR-329 expression in three nonneoplastic brain specimens and primary glioma tissues of nine individual patients. B ) Real-time PCR analysis of miR-329 expression in primary normal human astrocytes (NHA) and glioma cell lines (including A172, LN340, U118MG, LN464, SNB19, LN18, T98G, and U251MG). The average miR-329 expression was normalized by U6 expression. Each bar represents the mean of three independent experiments. * P < 0.05. C ) Western blotting analysis of E2F1 in primary normal human astrocytes (NHA) and glioma cell lines (including A172, LN340, U118MG, LN464, SNB19, LN18, T98G, and U251MG), β-actin served as the loading control.