Figure 5

mRNA-electroporated moDCs and LCs retain eGFP expression after cryopreservation. Immature (□) and partially-matured (▤) moDCs and LCs were electroporated with eGFP mRNA and cryopreserved. After thawing, eGFP expression for each experimental group was measured by flow cytometry and compared with pre-cryopreservation eGFP expression values to determine percent retention of eGFP expression. Pooled data are shown for each experimental group (mean ± SD, n = 3 independent experiments, ns = not significant).