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Figure 3 | Journal of Translational Medicine

Figure 3

From: Langerhans-type and monocyte-derived human dendritic cells have different susceptibilities to mRNA electroporation with distinct effects on maturation and activation: implications for immunogenicity in dendritic cell-based immunotherapy

Figure 3

mRNA electroporation induces the maturation and activation of LCs to a greater magnitude than for moDCs. Immature moDCs and LCs were electroporated with eGFP mRNA and then cultured with (+) or without (−) standard maturation-inducing inflammatory cytokines. After 24 hours, cells in each experimental group were compared with pre-electroporation controls by flow cytometry for the expression of phenotypic markers of DC maturation and activation, based on the upregulation of (A) CD83, (B) CD80, and (C) CD86, respectively. Representative dot plots of eGFP mRNA-electroporated moDCs and LCs from one of three independent experiments are shown in the top row. Pooled data for each experimental group (mean ± SD, n = 3 independent experiments) are shown in the bottom row (gray bar = pre-electroporation control, white bar = post-electroporation without inflammatory cytokines, black bar = post-electroporation with inflammatory cytokines). *P < .05, **P < .01, ***P < .001, relative to pre-electroporation control, except where brackets indicate comparisons between post-electroporation groups.

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