The maturation status of moDCs and LCs affects mRNA-electroporation transfection efficiency. Immature (□) and partially-matured (▤) moDCs (A, C) and LCs (B, D) were electroporated with eGFP-encoding mRNA. The transfection efficiency for each experimental group was assessed by flow cytometry at the indicated time points. Pooled data (mean ± SD, n = 6 independent experiments) are shown for moDCs (A) and LCs (B). *P < .05 and **P < .01 for comparisons between immature and mature groups. Representative histograms of eGFP mRNA-electroporated immature moDCs (C) and partially-matured LCs (D) from one of six independent experiments are shown.