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Table 4 VARI RNA and pathological QC

From: A multi-site feasibility study for personalized medicine in canines with Osteosarcoma

Patient ID RNA QC Pathology QC
A260/280 RIN % Viable tumor1 % Viable non-Neoplastic tissue1 % Necrotic tissue VARI diagnosis Clinical site diagnosis
  (1st Cut / 2nd Cut)
AH-301 2.27 8 75/75 5/10 20/15 Chondrosarcoma Chondrosarcoma
FS 201 2.08 7.8 80/80 20/20 15/25 Poorly differentiated tumor. Atypical Osteosarcoma OSA
RV 281 2.11 9.3 75/75 20/20 10/10 Osteosarcoma Chronic suppurative inflammation
MH 101 1.84 5.52     No Pathology due to RNA QC failure OSA
TL-141 2.11 6.8 60/50 30/50 10/0 Osteosarcoma. OSA
RB-181 2.11 7.5 80/85 10/5 10/10 Osteosarcoma OSA
RB-182 2.07 8.5 60/60 20/30 20/10 Osteosarcoma OSA
RB-183 2.09 6.8 90/80 10/10 0/10 Osteosarcoma (FFPE) OSA
VS 121 2.06 6.4 90/95 5/0 5/5 Osteosarcoma with a differential diagnosis of undifferentiated sarcoma. OSA
AZ-221 2.09 8.1 50/50 50/40 0/10 Osteosarcoma (FFPE) OSA
RV-282       Sample lost due to shipping delay. OSA
NC-01 2.1 8 80/80 15/10 5/10 Osteosarcoma. OSA
FS 202 2.09 8.53 60/65 30/25 10/10 Osteosarcoma. (Frozen and FFPE) OSA
FS 203 2.03 7.2 No evidence of tumor, values not recorded No evidence of tumor (Frozen and FFPE) Chronic inflammation   
RB 184 2.15 6.84 65/70 30/30 5/0 Osteosarcoma. OSA
RB 185 1.8 6.9 90/75 5/20 5/15 Osteosarcoma. (FFPE) OSA
RB 186 2.11 5.42     No Pathology due to RNA QC failure OSA
RV 283 2.09 8.4 60/60 40/40 0/0 Undifferentiated sarcoma (FFPE). OSA
NC 162 2.08 7.4 0/0 70/70 30/30 Normal tissue. Not available
RB 187 2.09 8.63 65/65 25/25 10/10 Osteosarcoma Suspected Synovial carcinoma
  1. 1 - Scores based on % nuclei.
  2. 2 - Failed RIN.
  3. 3 No RIN generated by Agilent software, even though sample visually looks good. Altered Fast lane threshold (to 1) to obtain RIN.
  4. 4 Sample shipped without VARI RIN analysis, value given determined at CRL.