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Table 4 VARI RNA and pathological QC

From: A multi-site feasibility study for personalized medicine in canines with Osteosarcoma

Patient ID

RNA QC

Pathology QC

A260/280

RIN

% Viable tumor1

% Viable non-Neoplastic tissue1

% Necrotic tissue

VARI diagnosis

Clinical site diagnosis

 

(1st Cut / 2nd Cut)

AH-301

2.27

8

75/75

5/10

20/15

Chondrosarcoma

Chondrosarcoma

FS 201

2.08

7.8

80/80

20/20

15/25

Poorly differentiated tumor. Atypical Osteosarcoma

OSA

RV 281

2.11

9.3

75/75

20/20

10/10

Osteosarcoma

Chronic suppurative inflammation

MH 101

1.84

5.52

   

No Pathology due to RNA QC failure

OSA

TL-141

2.11

6.8

60/50

30/50

10/0

Osteosarcoma.

OSA

RB-181

2.11

7.5

80/85

10/5

10/10

Osteosarcoma

OSA

RB-182

2.07

8.5

60/60

20/30

20/10

Osteosarcoma

OSA

RB-183

2.09

6.8

90/80

10/10

0/10

Osteosarcoma (FFPE)

OSA

VS 121

2.06

6.4

90/95

5/0

5/5

Osteosarcoma with a differential diagnosis of undifferentiated sarcoma.

OSA

AZ-221

2.09

8.1

50/50

50/40

0/10

Osteosarcoma (FFPE)

OSA

RV-282

     

Sample lost due to shipping delay.

OSA

NC-01

2.1

8

80/80

15/10

5/10

Osteosarcoma.

OSA

FS 202

2.09

8.53

60/65

30/25

10/10

Osteosarcoma. (Frozen and FFPE)

OSA

FS 203

2.03

7.2

No evidence of tumor, values not recorded

No evidence of tumor (Frozen and FFPE)

Chronic inflammation

  

RB 184

2.15

6.84

65/70

30/30

5/0

Osteosarcoma.

OSA

RB 185

1.8

6.9

90/75

5/20

5/15

Osteosarcoma. (FFPE)

OSA

RB 186

2.11

5.42

   

No Pathology due to RNA QC failure

OSA

RV 283

2.09

8.4

60/60

40/40

0/0

Undifferentiated sarcoma (FFPE).

OSA

NC 162

2.08

7.4

0/0

70/70

30/30

Normal tissue.

Not available

RB 187

2.09

8.63

65/65

25/25

10/10

Osteosarcoma

Suspected Synovial carcinoma

  1. 1 - Scores based on % nuclei.
  2. 2 - Failed RIN.
  3. 3 No RIN generated by Agilent software, even though sample visually looks good. Altered Fast lane threshold (to 1) to obtain RIN.
  4. 4 Sample shipped without VARI RIN analysis, value given determined at CRL.