Table 1 Primers for reverse transcription of microRNAs
No. | Assay name | Gene Bank/miRbase Access No. | Primers for reverse transcriptase Sequence(5' to 3') |
|---|---|---|---|
1 | cfa-miR-451 | MIMAT0009870 | AAACCGUUACCAUUACUGAGUU |
2 | cfa-miR-423 | MIMAT0006742 | UGAGGGGCAGAGAGCGAGACUUU |
3 | cfa-miR-23 | MIMAT0006640 | AUCACAUUGCCAGGGAUUU |
4 | cfa-miR-133 | MIMAT0009835 | UUUGGUCCCCUUCAACCAGCUA |
5 | cfa-miR-489 | MIMAT0009860 | GUGACAUCACAUAUACGGCGGC |
6 | cfa-miR-574 | MIMAT0006673 | CACGCUCAUGCACACACCCACA |
7 | cfa-miR-214 | MIMAT0009847 | ACAGCAGGCACAGACAGGCAGU |
8 | cfa-miR-1 | MIMAT0006656 | UGGAAUGUAAAGAAGUAUGUA |
9 | cfa-miR-494 | MIMAT0009905 | UGAAACAUACACGGGAAACCUC |
10 | cfa-miR-499 | MIMAT0006655 | UUAAGACUUGCAGUGAUGUUU |
Control | human-RNU6B | NR_002752 | Â |