Figure 7

Protein expression levels of survivin in resting and activated CD8+ T cells. CD8+ T cells from three healthy donors were isolated and cultured for 48 hours. Half of the T cells per donor were kept in culture medium (resting T cells), the other half were activated with PHA and IL-2 (activated T cells). A) Representative FACS histogram of resting T cells stained for the activation marker CD25. About 5% of the plotted cells are CD25 positive. B) Representative FACS histogram of activated T cells stained for the activation marker CD25. About 91% of the plotted cells are CD25 positive. C) Western blot of resting (−) or activated (+) T cells from three different donors (A, B, C), stained with anti-survivin antibodies. The arrow indicates survivin protein as a band of around 16.3 kD. Household β-actin protein staining was used as a loading control.