Figure 4

Tetramer staining after T cell induction. A) CD8+ T cells from donor 1, were stimulated twice with autologous mature DC loaded with either Influenza peptide or electroporated with mRNA encoding Ubi-Mart4. The T cells were stained with an irrelevant tetramer (HPV E7 11), Influenza 58 and Mart-1 27 L in APC (X-axis) and PE (Y-axis). T cells plotted in the upper right quadrant were considered antigen specific. B) CD8+ T cells from donor 2, were stimulated five times with autologous DC electroporated with mRNA encoding full length survivin. The T cells were stained with an irrelevant tetramer (Her2/neu 369), survivin 96 and survivin 96 M in APC (X-axis) and PE (Y-axis). T cells plotted in the upper right quadrant were considered antigen specific.