Figure 3

Survivin expression after electroporation of mRNA in mature dendritic cells. A) Mature monocyte derived dendritic cells were electroporated with mRNA encoding eGFP, full length survivin or full length Ubi-survivin. Four hours after electroporation the DCs were harvested and lysed. Samples contained equal amounts of cellular protein. The arrow indicates a protein band of 16.3 kD, the predicted molecular weight of full length survivin. B) Cytospin preparation were obtained from mature monocyte derived dendritic cells electroporated with mRNA encoding either eGFP (upper panel), full length survivin (middle panel) or full length Ubi-survivin (lower panel). After 2, 4, 6, 8 and 24 hours the electroporated DCs were harvested, spun down on a glass slide and stained with anti-survivin antibodies. Brown staining indicates survivin protein. Nuclei appear in blue.