Effects of Treg-inactivation on the efficacy of DC-tumor cell fusion vaccination. Lung metastases were established in C57BL/6 mice via tail vein injection of D5lacZ cells. After 2 days, mice received intraperitoneal injections of saline (−), Y13 mAb (Y13), or PC61 mAb (PC61). On day 3, DC-tumor fusion cells (FC) were delivered intranodally. IL-12 was administered intraperitoneally daily for 4 days, starting on the day of vaccination. Mice were sacrificed and lung metastases were enumerated after 21 days. P-values were calculated using the two-tailed Wilcoxon rank sum test. The number (n) of animals used in each group is indicated. Results are presented as box plots in which the median, the 25 percentile, the 75 percentile, as well as minimum and maximum of each group are shown.