Dexmedetomidine inhibited the phosphorylations of JAK2, STAT1 and STAT3. Representative Western blots for the phosphorylations of JAK2, STAT1, STAT3 and total JAK2, STAT1, STAT3 expression (A) of the kidneys were detected after 48 h of renal I/R in the sham, IRI, DMSO, Atip, DEX and AG490 groups. Densitometry analysis of Western blots for the ratio of p-JAK2/JAK2 (B), p-STAT1/STAT1 (C) and p-STAT3/STAT3 (D). Data were represented as mean ± SEM (n = 8). *P < 0.05 and **P < 0.01 vs. the sham group. #P < 0.05 vs. the IRI and DMSO groups. $P < 0.05 vs. the DEX group.