PTK6 overexpression simulates the proliferation and transforming ability of NPC cells. (A) PTK6 overexpression in HNE1 cells at 36 hours post-transfection. HNE1 cells were transfected with pcDNA3.1-PTK6 and the control plasmid pcDNA3.1-empty vector, respectively. A dramatically increased mRNA level of PTK6 was detected in pcDNA3.1-PTK6 transfected cells by quantitative RT-PCR. (B) Western blotting analysis showed that the protein level of PTK6 in HNE1 cells after transfection. (C) The growth curves of HNE1 cell line. After transient transfection of PTK6, the PTK6-overexpression cells grew significantly faster than control cells from day 2 to day 5 (P<0.001, marked by *). Error bar =SD. (D) Representative pictures of colony formation assay of the HNE1 cell line transfected with pcDNA3.1-PTK6 and the control plasmid pcDNA3.1-empty vector. Upon the transient transfection expression of PTK6, HNE1 cells formed more colonies compared to the control cells. Error bar =SD.