Figure 4

Modulation of DNA methylation and histone methylation by Adox in human adult erythroid progenitor cells. (A) Histone H4R3me2s ChIP analysis of γ-gene promoter from human BM cells treated with either PBS control, Adox or decitabine as in Figure 3C. Graphs show mean ± SD, n = 3. (B) Histone H4R3me2s ChIP analysis of CDH1 promoter from human BM cells treated with either PBS control or Adox as in Figure 3C. Graphs show mean ± SD, n = 3. (C) Histone H4 acetylation ChIP analysis of γ-promoter from human BM cells treated with either PBS control or Adox as in Figure 3C. Graphs show mean ± SD, n = 3. (D) Quantitation of DNA methylation at the human γ-globin gene in human BM cells treated as in (A). The numbers on the bottom represent the positions of the CpG dinucleotides relative to the transcriptional start site of the γ-gene. Ave indicates average methylation of the 4 total CpGs. *P < 0.05, #P > 0.05 using Chi-squared test.