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Figure 2 | Journal of Translational Medicine

Figure 2

From: Low-dose temozolomide before dendritic-cell vaccination reduces (specifically) CD4+CD25++Foxp3+ regulatory T-cells in advanced melanoma patients

Figure 2

Flow cytometry detection of CD4 + CD25 ++ . (A) A dot plot of forward scatter (FSC) and side scatter (SSC) was used to define the lymphocyte population (P1). (B-C) The expression of CD4 and CD25 total lymphocytes (P1) was detected and compared with that of the negative control and different gates were drawn to define CD4+CD25_ cells (P2), CD4+CD25+low-medium cells (P3) and CD4+CD25++ cells (P4). The percentage of CD4+CD25++FoxP3+ cells in total lymhocytes was determinated. (D-E) The histograms show the percentage of FoxP3+Treg cells compared with the negative control.

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