Figure 4

Id1 regulates the expression of MMP-2 via NF-κB in EPCs. (A) MMP-2 and MMP-9 mRNA expression by real-time RT-PCR. Data are expressed as means ± S.E. **p < 0.01 vs. control, #, &, $p < 0.05 vs. ovarian cancer. (B) Typical western blot images showing protein expression of MMP-2 and MMP-9 (β-actin is shown as a housekeeping control). (C) The graph showing the relative MMP-2 and MMP-9 protein levels normalized to β-actin. The results are expressed as the mean ± S.E. **p < 0.01 vs. control, #,&,$p < 0.05 vs. ovarian cancer. (D) Transfection of EPCs with Id1 significantly increased the promotor activity of NF-κB by luciferase assay. Increased promotor activity was abrogated by PDTC. RLA = relative luciferase activity. *p < 0.05 vs. ovarian cancer, #p < 0.05 vs. Id1-LV.