Table 2 List of proteins identified from 2D gels of protein extracts of cardiac tissue
Spot no. | Category | Accession no. | Protein name | No. of matched peptides | Protein score | M.Wt | pI |
|---|---|---|---|---|---|---|---|
1. | Uncharacterised | gi|55628 | Unnamed protein product [rattus norvegicus] | 14 | 75 | 70670 | 6.09 |
2. | Transport | gi|149033753 | Albumin, isoform cra_a [rattus norvegicus] | 13 | 78 | 53060 | 6.72 |
3. | Transport | gi|149033753 | Albumin, isoform cra_a [rattus norvegicus] | 10 | 87 | 53060 | 6.72 |
4. | Chaperone | gi|1000439 +gi|178847300 | grp75 [rattus sp.] chain a, crystal structure of the 70-kda heat shock cognate protein | 31 | 110 | 73984 | 5.87 |
+59895 | 5.91 | ||||||
5. | Uncharacterised | gi|149041392 | rcg57965, isoform cra_b | 9 | 65 | 48623 | 6.16 |
6. | Signal transduction | gi|149022678 | Mitogen activated protein kinase 8 interacting protein, isoform cra_d | 6 | 47 | 75419 | 4.73 |
7. | Chaperone | gi|1000439 | grp75 [rattus sp.] | 10 | 72 | 73984 | 5.87 |
8. | Muscle contraction and development | gi|149058891 | Calpastatin, isoform cra_a | 4 | 43 | 20886 | 5.20 |
9. | Uncharacterised | gi|1334284 | Unnamed protein product | 8 | 47 | 58061 | 5.35 |
10. | Chaperone | gi|56383 | Heat shock protein (hsp60) precursor | 17 | 90 | 61098 | 5.91 |
11. | Uncharacterised | gi|1334284, | Unnamed protein product | 13 | 74 | 58061 | 5.35 |
12. | Muscle contraction and development | gi|11968118 | Desmin | 7 | 61 | 53481 | 5.21 |
13. | Muscle contraction and development | gi|11968118 | Desmin [rattus norvegicus] | 29 | 226 | 53481 | 5.21 |
14. | ATP biosynthesis | gi|1374715 | ATP synthase beta subunit | 32 | 159 | 51171 | 4.92 |
15. | ATP biosynthesis | gi|1374715 | ATP synthase beta subunit | 22 | 140 | 51171 | 4.92 |
16. | Uncharacterised | gi|293344961 | predicted: hypothetical protein | 7 | 45 | 32379 | 8.55 |
17. | TCA cycle | gi|149025181 | Dihydrolipoamide s-succinyltransferase (e2 component of 2-oxo-glutarate comp. | 7 | 55 | 21198 | 6.92 |
18. | TCA cycle | gi|149025181 | Dihydrolipoamide s-succinyltransferase (e2 component of 2-oxo-glutarate comp. | 5 | 55 | 21198 | 6.92 |
19. | Transport | gi|149033753 | Albumin, isoform cra_a | 13 | 87 | 53060 | 6.72 |
20. | Transport | gi|149033753 | Albumin, isoform cra_a | 14 | 83 | 53060 | 6.72 |
21. | Transport | gi|149033753 | Albumin, isoform cra_a | 13 | 82 | 53060 | 6.72 |
22. | Transport | gi|149033753 | Albumin, isoform cra_a | 13 | 78 | 53060 | 6.72 |
23. | Uncharacterised | gi|149066551 | rcg59557 | 5 | 54 | 12861 | 9.18 |
24. | Uncharacterised | gi|149063941 | rcg23467, isoform cra_a | 5 | 51 | 223560 | 5.59 |
25. | Muscle contraction and development | gi|6981240, | Myosin light chain 3 | 16 | 97 | 22256 | 5.03 |
26. | Muscle contraction and development | gi|6981240, | Myosin light chain 3 | 13 | 55 | 22256 | 5.03 |
27. | Protein, cholesterol metabolism | gi|149063506 | Phosphatidylethanolamine binding protein 1, isoform cra_a | 7 | 42 | 10775 | 6.26 |
28. | Protein, cholesterol metabolism, transport | gi|6978515 | Apolipoprotein a-i preproprotein | 3 | 43 | 30100 | 5.52 |
29. | DNA synthesis | gi|6679299 | Prohibitin | 5 | 141 | 29859 | 5.57 |
30. | Uncharacterised | gi|149054764 | rcg33654, isoform cra_d | 14 | 48 | 16329 | 5.45 |
31. | Ubiquitinisation | gi|149016294 | F-box only protein 36 (predicted), isoform cra_c | 4 | 40 | 9895 | 9.52 |
32. | Muscle contraction and development | gi|127167 | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | 6 | 120 | 18868 | 4.86 |
33. | Muscle contraction and development | mlrv_rat | mlrv_rat, myosin regulatory light chain 2, ventricular/cardiac muscle isoform | 12 | 169 | 18868 | 4.86 |
34. | Uncharacterised | gi|293349387 | Predicted: hcg2036631-like | 23 | 54 | 51880 | 9.69 |
35. | Protein,cholesterol metabolism | gi|149043202 | Calpain 9 | 7 | 43 | 51956 | 5.79 |
36. | Transport | gi|13162363 | Fatty acid-binding protein, heart | 9 | 96 | 14766 | 5.91 |
37. | Muscle contraction and development | gi|20302069 | Heat shock protein beta-6 | 9 | 68 | 17551 | 6.05 |